2022

Doublier, S., Cirrincione, S., Scardaci, R., Botta, C., Lamberti, C., Di Giuseppe, F.,Angelucci S, Rantsiou K, Cocolin L. & Pessione, E. (2022). Putative probiotics decrease cell viability and enhance chemotherapy effectiveness in human cancer cells: role of butyrate and secreted proteins. Microbiological Research, 260, 127012.

Scardaci, R., Bietto, F., Racine, P. J., Boukerb, A. M., Lesouhaitier, O., Feuilloley, M. G., Scutera S, Musso T, Connil N. & Pessione, E. (2022). Norepinephrine and serotonin can modulate the behavior of the probiotic Enterococcus faecium NCIMB10415 towards the host: Is a putative surface sensor involved? Microorganisms, 10(3), 487.

Famulari A, Correddu D, Nardo GD, Gilardi G, Chiesa M, GarcÌa-Rubio I. CYP116B5hd, a self-sufficient P450 cytochrome: A dataset of its electronic and geometrical properties. Data Brief. 2022 Apr 25;42:108195. doi:10.1016/j.dib.2022.108195.

Cheropkina H, Catucci G, Cesano F, Marucco A, Gilardi G, Sadeghi SJ. Bioelectrochemical platform with human monooxygenases: FMO1 and CYP3A4 tandem reactions with phorate. Bioelectrochemistry. 2022 Nov 23;150:108327. doi:10.1016/j.bioelechem.2022.108327.

Gea M, Zhang C, Tota R, Gilardi G, Di Nardo G, SchilirÚ T. Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase. Int J Environ Res Public Health. 2022 Feb 10;19(4):1959. doi:10.3390/ijerph19041959.

Famulari A, Correddu D, Di Nardo G, Gilardi G, Chiesa M, GarcÌa-Rubio I. EPR characterization of the heme domain of a self-sufficient cytochrome P450 CYP116B5). J Inorg Biochem. 2022 Jun;231:111785. doi:10.1016/j.jinorgbio.2022.111785.

Correddu D, Helmy Aly S, Di Nardo G, Catucci G, Prandi C, Blangetti M, Bellomo C, Bonometti E, Viscardi G, Gilardi G. Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives. RSC Adv. 2022 Nov 28;12(52):33964-33969. doi: 10.1039/d2ra06029a.

Giuriato D, Correddu D, Catucci G, Di Nardo G, Bolchi C, Pallavicini M, Gilardi G. Design of a H₂ O₂ -generating P450_SP fusion protein for high yield fatty acid conversion. Protein Sci. 2022 Dec;31(12):e4501. doi: 10.1002/pro.4501.

Zhang C, Gilardi G, Di Nardo G. Depicting the proton relay network in human aromatase: New insights into the role of the alcohol-acid pair. Protein Sci. 2022 Sep;31(9):e4389. doi: 10.1002/pro.4389.

Catucci G, Ciaramella A, Di Nardo G, Zhang C, CastrignanÚ S, Gilardi G. Molecular Lego of Human Cytochrome P450: The Key Role of Heme Domain Flexibility for the Activity of the Chimeric Proteins. Int J Mol Sci. 2022 Mar 25;23(7):3618. doi: 10.3390/ijms23073618.

2021

Pessione E. and Garcia Contreras R. Non-conventional antimicrobial agents. In: “Encyclopedia of infection and immunity” Elsevier 2021

Bosco F, Cirrincione S, Carletto R, Marmo L, Chiesa F, Mazzoli R and Pessione E (2021). PHA Production from Cheese Whey and “Scotta”: Comparison between a Consortium and a Pure Culture of Leuconostoc mesenteroides. Microorganisms, 9 (12), 2426.

Cheropkina H, Catucci G, Marucco A, Fenoglio I, Gilardi G, Sadeghi SJ. Human flavin-containing monooxygenase 1 and its long-sought hydroperoxyflavin intermediate. Biochem Pharmacol. 2021, 193, 114763. doi: 10.1016/j.bcp.2021.114763.

Di Nardo G, Di Venere A, Zhang C, Nicolai E, Castrignanò S, Di Paola L, Gilardi G, Mei G. Polymorphism on human aromatase affects protein dynamics and substrate binding: spectroscopic evidence. Biol Direct. 2021 Apr 26;16(1):8. doi: 10.1186/s13062-021-00292-9.

Costa P, Usai G, Re A, Manfredi M, Mannino G, Bertea CM, Pessione E, Mazzoli R. 2021. Clostridium cellulovorans proteomic responses to butanol stress. Frontiers in Microbiology 12, 1881.

Scardaci R., Manfredi M., Scutera S., Marengo E, Pessione E. 2021 Serotonin exposure improves stress resistance and inter-bacterial interactions in the probiotic Enterococcus faecium NCIMB10415. Microbiology Research, 12, 606-625.

Cirrincione S., Luganini A., Lamberti C., Manfredi M., Cavallarin L. Giuffrida MG and Pessione E. 2021. Antiviral and antibacterial peptides produced by L. lactis subsp. cremoris and L. rhamnosus from donkey milk. Molecules, 26 (16) 5100.

Correddu, D.; Di Nardo, G.; Gilardi, G. Self-Sufficient Class VII Cytochromes P450: From Full-Length Structure to Synthetic Biology Applications. Trends in Biotechnology 2021, S0167779921000329, doi:10.1016/j.tibtech.2021.01.011.

Winkler, M.; Duan, J.; Rutz, A.; Felbek, C.; Scholtysek, L.; Lampret, O.; Jaenecke, J.; Apfel, U.-P.; Gilardi, G.; Valetti, F.; et al. A Safety Cap Protects Hydrogenase from Oxygen Attack. Nat Commun 2021, 12, 756, doi:10.1038/s41467-020-20861-2.

Di Nardo, G.; Zhang, C.; Marcelli, A.G.; Gilardi, G. Molecular and Structural Evolution of Cytochrome P450 Aromatase. IJMS 2021, 22, 631, doi:10.3390/ijms22020631.

Laroute, V.; Mazzoli, R.; Loubière, P.; Pessione, E.; Cocaign-Bousquet, M. Environmental Conditions Affecting GABA Production in Lactococcus Lactis NCDO 2118. Microorganisms 2021, 9, 122, doi:10.3390/microorganisms9010122.

Nebbia, S.; Lamberti, C.; Lo Bianco, G.; Cirrincione, S.; Laroute, V.; Cocaign-Bousquet, M.; Cavallarin, L.; Giuffrida, M.G.; Pessione, E. Antimicrobial Potential of Food Lactic Acid Bacteria: Bioactive Peptide Decrypting from Caseins and Bacteriocin Production. Microorganisms 2020, 9, 65, doi:10.3390/microorganisms9010065.

Panicco, P.; Castrignanò, S.; Sadeghi, S.J.; Nardo, G.D.; Gilardi, G. Engineered Human CYP2C9 and Its Main Polymorphic Variants for Bioelectrochemical Measurements of Catalytic Response. Bioelectrochemistry 2021, 138, 107729, doi:10.1016/j.bioelechem.2020.107729.

Pessione, E. The Less Expensive Choice: Bacterial Strategies to Achieve Successful and Sustainable Reciprocal Interactions. Front. Microbiol. 2021, 11, 571417, doi:10.3389/fmicb.2020.571417.

Scardaci, R.; Varese, F.; Manfredi, M.; Marengo, E.; Mazzoli, R.; Pessione, E. Enterococcus Faecium NCIMB10415 Responds to Norepinephrine by Altering Protein Profiles and Phenotypic Characters. Journal of Proteomics 2021, 231, 104003, doi:10.1016/j.jprot.2020.104003.

Bortolussi, S.; Catucci, G.; Gilardi, G.; Sadeghi, S.J. N- and S-Oxygenation Activity of Truncated Human Flavin-Containing Monooxygenase 3 and Its Common Polymorphic Variants. Archives of Biochemistry and Biophysics 2021, 697, 108663, doi:10.1016/j.abb.2020.108663.

2020

Zhang C., Schilirò T., Gea M., Bianchi, S. Spinello A., Magistrato A., Gilardi G, Di Nardo G. Molecular Basis for Endocrine Disruption by Pesticides Targeting Aromatase and Estrogen Receptor. Int J Environ Res Public Health. (2020) 17(16): 5664. Published online, doi: 10.3390/ijerph17165664.

Zhang C., Catucci G., Di Nardo G., Gilardi G.. Effector role of cytochrome P450 reductase for androstenedione binding to human aromatase. International Journal of Biological Macromolecules. (2020) 164, pp. 510-517.

Catucci,G., Aramini D., Sadeghi S.J., Gilardi G. Ligand stabilization and effect on unfolding by polymorphism in human flavin-containing monooxygenase 3. International Journal of Biological Macromolecules. 2020, 162, 1484-1493.

Di Nardo G. Gilardi G. Natural Compounds as Pharmaceuticals: The Key Role of Cytochromes P450 Reactivity. Trends Biochem Sci. (2020) 45(6):511-525. doi: 10.1016/j.tibs.2020.03.004.

Usai G., Cirrincione S., Re A., Manfredi M., Pagnani, A., Pessione E., Mazzoli R. 2020 Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach. Journal of Proteomics (2020) 216, 103667. doi.org/10.1016/j.jprot.2020.103667

Pessione E. 2020.The Russian doll model: how bacteria shape successful and sustainable interkingdom relationships. Frontiers in Systems Microbiology (2020) doi: 10.3389/fmicb.2020.573759.

Catucci G., Valetti F., Sadeghi, S.J., Gilardi, G. Biochemical features of dye-decolorizing peroxidases: Current impact on lignin degradation. Biotechnology and Applied Biochemistry, 2020, 67(5), 751-759.

Sadeghi S.J., Di Nardo G., Gilardi, G. Chimeric cytochrome P450 3A4 used for in vitro prediction of food–drug interaction. Biotechnology and Applied Biochemistry, 2020, 67(4), 541-548.

Catucci G., Gilardi G., Sadeghi S.J. Production of drug metabolites by human FMO3 in Escherichia coli. Microb Cell Fact. 2020 19(1):74. doi: 10.1186/s12934-020-01332-1.

Somale D., Di Nardo G., di Blasio L., Puliafito A., Vara-Messler M., Chiaverina G., Palmiero M., Monica V., Gilardi G., Primo L., Gagliardi P.A. Activation of RSK by phosphomimetic substitution in the activation loop is prevented by structural constraints. Sci Rep. 2020 10(1):591. doi: 10.1038/s41598-019-56937-3.

Ciaramella A., Catucci G., Di Nardo G., Sadeghi S.J., Gilardi G. Peroxide-driven catalysis of the heme domain of A. radioresistens cytochrome P450 116B5 for sustainable aromatic rings oxidation and drug metabolites production. N Biotechnol. (2020) 25, 54:71-79. doi: 10.1016/j.nbt.2019.08.005.

Parween S, Di Nardo G, Baj F, Zhang C, Gilardi G, Pandey AV. Differential effects of variations in human P450 oxidoreductase on the aromatase activity of CYP19A1 polymorphisms R264C and R264H. J Steroid Biochem Mol Biol. (2020) 196, 105507. doi: 10.1016/j.jsbmb.2019.105507.

Minerdi D, Sadeghi SJ, Pautasso L, Morra S, Aigotti R, Medana C, Gilardi G, Gullino ML, Gilardi G. Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae. Biochim Biophys Acta Proteins Proteom. (2020) 140268. doi: 10.1016/j.bbapap.2019.140268.

2019

Catucci, G., Sadeghi, S.J., Gilardi, G. A direct time-based ITC approach for substrate turnover measurements demonstrated on human FMO3. Chem Commun (Camb).  (2019), 55, 6217-6220. doi: 10.1039/c9cc01356c.

Catucci G, Gao C, Rampolla G, Gilardi G, Sadeghi SJ.Uncoupled human flavin-containing monooxygenase 3 releases superoxide radical in addition to hydrogen peroxide. Free Radic Biol Med. (2019), 145:250-255. doi: 10.1016/j.freeradbiomed.2019.09.038.

Parween S, Di Nardo G, Baj F, Zhang C, Gilardi G, Pandey AV. Differential effects of variations in human P450 oxidoreductase on the aromatase activity of CYP19A1 polymorphisms R264C and R264H. J Steroid Biochem Mol Biol. (2019) 196, 105507. doi: 10.1016/j.jsbmb.2019.105507.

Ciaramella A, Catucci G, Gilardi G, Di Nardo G. Crystal structure of bacterial CYP116B5 heme domain: New insights on class VII P450s structural flexibility and peroxygenase activity. Int J Biol Macromol. (2019) 140:577-587. doi:10.1016/j.ijbiomac.2019.08.141.

Nebbia, S., Lamberti, C., Giorgis, V., Giuffrida, M. G., Manfredi, M., Marengo, E., ... & Cavallarin, L. (2019). The Cockroach Allergen‐like protein is involved in primary respiratory and food allergy to yellow mealworm (Tenebrio molitor). Clinical & Experimental Allergy. doi: 10.1111/cea.13461

Porto, B. A., Monteiro, C. F., Souza, É. L., Leocádio, P. C., Alvarez-Leite, J. I., Generoso, S. V., ... & Nicoli, J. R. (2019). Treatment with selenium-enriched Saccharomyces cerevisiae UFMG A-905 partially ameliorates mucositis induced by 5-fluorouracil in mice. Cancer chemotherapy and pharmacology, 84(1), 117-126. doi: 10.1007/s00280-019-03865-8

Cirrincione, S., Neumann, B., Zuehlke, D., Riedel, K., & Pessione, E. (2019). Detailed sub-proteome analyses of a food-isolated Enterococcus faecalis provide insights into food safety and potential probiotic value. Frontiers in Nutrition, 6, 71. doi: 10.3389/fnut.2019.00071

Vaudano, E., Quinterno, G., Costantini, A., Pulcini, L., Pessione, E., & Garcia-Moruno, E. (2019). Yeast distribution in Grignolino grapes growing in a new vineyard in Piedmont and the technological characterization of indigenous Saccharomyces spp. strains. International journal of food microbiology, 289, 154-161. doi: 10.1016/j.ijfoodmicro.2018.09.016

2018

Luganini, A., Di Nardo, D., Munaron, L., Gilardi, G., Pla, A.F. and Gribaudo, G. “Human cytomegalovirus US21 protein is a viroporin that modulates calcium homeostasis and protects cells against apoptosis”. PNAS (2018), 115, E12370-E12377. DOI: 10.1073/pnas.1813183115

Mazzoli R, Giuffrida MG, Pessione E. “Back to the past. 3 Forever young: cutting-edge biochemical and microbiological tools for cultural heritage conservation” 2018 Appl. Microbiol. Biotechnol. 102, pages6815–6825. doi: 10.1007/s00253-018-9121-3.

Cirrincione S., Breuer Y., Mangiapane E., Mazzoli R.,  Pessione E. “'Ropy' phenotype, exopolysaccharides and metabolism: study on food isolated potential probiotics LAB. 2018 Microbiol Res. 214: 137-145. doi: 10.1016/j.micres.2018.07.004.

Bruni, N., Della Pepa, C., Oliaro-Bosso, S., Pessione, E., Gastaldi, D., & Dosio, F. (2018). Cannabinoid delivery systems for pain and inflammation treatment. Molecules, 23(10), 2478.

Catucci, G., Bortolussi, S., Rampolla, G., Cusumano, D., Gilardi, G. and Sadeghi, S.J. “Flavin-Containing Monooxygenase 3 Polymorphic Variants Significantly Affect Clearance of Tamoxifen and Clomiphene”. Basic Clin Pharmacol Toxicol. (2018), 123, 687-691. DOI: 10.1111/bcpt.13089

Castrignanò, S., Di Nardo, G., Sadeghi, S.J. and Gilardi, G. “Influence of inter-domain dynamics and surrounding environment flexibility on the direct electrochemistry and electrocatalysis of self-sufficient cytochrome P450 3A4-BMR chimeras”. J Inorg Biochem (2018), 188, 9-17. DOI: 10.1016/j.jinorgbio.2018.08.003

Gao, C., Catucci, G., Gilardi, G. and Sadeghi, S.J. “Binding of methimazole and NADP(H) to human FMO3: In vitro and in silico studies”. Inter J Biol Macromol (2018), 118, 460-468. DOI: 10.1016/j.ijbiomac.2018.06.104

Luganini, A., Terlizzi, M.E., Catucci, G., Gilardi, G., Maffei, M.E. and Gribaudo, G. “The cranberry extract oximacro® exerts in vitro virucidal activity against influenza virus by interfering with hemagglutinin”. Frontiers Microbiol (2018), 9, 1826. DOI: 10.3389/fmicb.2018.01826

Castrignanò, S., D'Avino, S., Di Nardo, G., Catucci, G., Sadeghi, S.J. and Gilardi, G.  “Modulation of the interaction between human P450 3A4 and B. megaterium reductase via engineered loops” BBA - Proteins and Proteomics (2018), 1866(1), 116-125DOI: 10.1016/j.bbapap.2017.07.009

Baravalle, R., Ciaramella, A., Baj, F., Di Nardo, G. and Gilardi, G.“Identification of endocrine disrupting chemicals acting on human aromatase” BBA - Proteins and Proteomics (2018), 1866(1), 88-96. DOI: 10.1016/j.bbapap.2017.05.013

Di Nardo, G., Cimicata, G., Baravalle, R., Dell'Angelo, V., Ciaramella, A., Catucci, G., Ugliengo, P. and Gilardi, G.  “Working at the membrane interface: Ligand-induced changes in dynamic conformation and oligomeric structure in human aromatase” Biotechnolology and Applied Biochemistry, (2018), 65, 46-53 DOI: 10.1002/bab.1613

Celebioglu, H. U., Delsoglio, M., Brix, S., Pessione, E., Svensson, B, Plant polyphenols stimulate adhesion to intestinal mucosa and induce proteome changes in the probiotic Lactobacillus acidophilus NCFM. Mol. Nutr .Food Res. (2018) doi: 10.1002/mnfr.201700638.

Noti O., Vaudano E., , Giuffrida M.G., Lamberti C., Cavallarin L., Garcia-Moruno E., Pessione E. Enhanced arginine biosynthesis and lower proteolytic profile as indicators of Saccharomyces cerevisiae stress in stationary phase during fermentation of high sugar grape must: A proteomic evidence 2018 Food Res. Int. 105, 1011-1018.

Giuffrida MG, Mazzoli R, Pessione E. “Back to the past. 1. Decyphering cultural heritage secrets by protein identification”. 2018 AMAB Accepted for publication DOI: 10.1007/s00253-018-8963-z

Mazzoli R, Giuffrida MG, Pessione E. “Back to the past. 2. Find the guilty: microorganisms involved in the biodeterioration of archeological and historical items" 2018 AMAB 102(16):6815-6825. doi: 10.1007/s00253-018-9121-3.

2017

Calderini E., Celebioglu H.U., Villarroel J., Jacobsen S., Svenson B., Pessione E “Oxidative stress responses of L.acidophilus NCFM involve DNA repair and cysteine de novo synthesis, as revealed by comparative proteomics”. Proteomics 2017 Mar;17(5). doi: 10.1002/pmic.201600178.

Mazzoli R, Riedel K, Pessione E. Editorial: Bioactive compounds from microbes. Front Microbiol. 2017 Mar 8;8:392. doi: 10.3389/fmicb.2017.00392. eCollection 2017.

Ferrari M., Mazzoli R., Morales S., Fedi M.E., Liccioli L., Piccirillo A., Cavaleri T., Oliva C., Gallo P., Borla M., Cardinali M and Pessione E. “Enzymatic laundry for old clothes: characterizing and biocleaning of an ancient coptic tunic” 2017 Appl. Microbiol and Biotechnol. 101:7041–7052, DOI 10.1007/s00253-017-8437-8

Gandini C., Tarraran L., Kalemasi D., Pessione E. and Mazzoli R. Recombinant Lactococcus lactis for efficient conversion of cellooligosaccharides cellodextrins into L-lactic acid. Biotech and Bioeng. 2017 114(12):2807-2817. doi: 10.1002/bit.26400

Gao, C., Catucci, G., Castrignanò, S., Gilardi, G. and Sadeghi, S.J. “Inactivation mechanism of N61S mutant of human FMO3 towards trimethylamine” Scientific Reports (2017), 7: 14668, DOI: 10.1038/s41598-017-15224-9

Catucci, G., Polignano, I., Cusumano, D., Medana, C., Gilardi, G., Sadeghi, S.J. “Identification of human flavin-containing monooxygenase 3 substrates by a colorimetric screening assay” Analytical Biochemistry, (2017) 522, 46-52, DOI: 10.1016/j.ab.2017.01.024

Degregorio, D., D'Avino, S., Castrignanò, S., di Nardo, G., Sadeghi, S.J., Catucci, G. and Gilardi, G. “Human cytochrome P450 3A4 as a biocatalyst: Effects of the engineered linker in modulation of coupling efficiency in 3A4-BMR chimeras” Frontiers in Pharmacology, (2017), 8 (MAR), art. no. 121, DOI: 10.3389/fphar.2017.00121

Baravalle, R., Di Nardo, G., Bandino, A., Barone, I., Catalano, S., Andò, S., Gilardi, G. "Impact of R264C and R264H polymorphisms in human aromatase function", Journal of Steroid Biochemistry & Molecular Biology, (2017), 23-32, DOI: 10.1016/j.jsbmb.2016.09. 022

Castrignanò, S., Bortolussi, S., Catucci, G., Gholami, O., Valetti, F., Gilardi, G., Sadeghi, S.J. “Bioelectrochemical profiling of two common polymorphic variants of human FMO3 in presence of graphene oxide” Electrochimica Acta, (2017) 228, 611-618, DOI: 10.1016/j.electacta.2017.01.131

Baravalle, R., Valetti, F., Catucci, G., Gambarotta, G., Chiesa, M., Maurelli, S., Giamello, E., Barone, I. Catalano, S. Andò, S., Di Nardo, G. and Gilardi, G., " Effect of sildenafil on human aromatase activity: from in vitro structural analysis to catalysis and inhibition in cells", Journal of Steroid Biochemistry & Molecular Biology, (2017), 165, 438-447, DOI: 10.1016/j.jsbmb.2016.09.003

2016

Ciaramella, A., Minerdi, D. and Gilardi, G. “Catalytically self-sufficient cytochromes P450 for green production of fine chemicals” Rendiconti Lincei, (2016), in press DOI 10.1007/s12210-016-0581-z

Di Nardo, G., Sadeghi, S.J. and Gilardi, G. "Utilizzo di monossigenasi per la biocatalisi industriale", La Chimica e L'Industria, (2016), 5, 52–55, DOI: 10.17374/CI.2016.98.5.

Morra, S., Arizzi, M., Valetti, F. and Gilardi, G. "Oxygen stability in the new [FeFe]-hydrogenase from Clostridium beijerinckii SM10 (CbA5H)", Biochemistry, (2016) DOI: 10.1021/acs.biochem.6b00780

Gilardi, G., Di Nardo, G., "Heme iron centers in Cytochrome P450: structure and catalytic activity", Rendiconti Lincei, (2016), in press, DOI: 10.1007/s12210-016-0565-z

Gao C, Catucci G, Di Nardo G, Gilardi G, Sadeghi SJ. Human flavin-containing monooxygenase 3: Structural mapping of gene polymorphisms and insights into molecular basis of drug binding. Gene. (2016), 593: 91-9. doi: 10.1016/j.gene.2016.08.020.

Arizzi M, Morra S, Pugliese M, Gullino ML, Gilardi G, Valetti F. Biohydrogen and biomethane production sustained by untreated matrices and alternative application of compost waste. Waste Manag. 2016 Oct;56:151-7. doi: 10.1016/j.wasman.2016.06.039.

Bruni N, Capucchio MT, Biasibetti E, Pessione E., Cirrincione S., Giraudo L, Corona A., Dosio F. Antimicrobial activity of lactoferrin-related peptides and applications in human and veterinary medicine". Molecules (2016) 21, 752-777.

Pessione E. Cirrincione S.: “Bioactive compounds released in food by LAB: biogenic amines and encrypted peptides” Frontiers in Systems Microbiol. (2016) 7, DOI: 10.3389/fmicb.2016.00876.

Laroute V, Mazzoli R., Yasaro C., Narin. W, Pessione E., Cocaign-Bousquet M, Loubière P.: GABA production in Lactococcus lactis is enhanced by Arginine and co-addition of malate (2016). Frontiers in Microbial Physiology and Metabolism (2016) 7, 1050

Vera-Pingitore E., Pessione A., Fontana C., Mazzoli R., Pessione E. Comparative proteomic analyses for elucidating metabolic changes during EPS production under different fermentation temperatures by Lactobacillus plantarum Q823”. Int. J. Food Micro. (2016) 238,96-102

Mazzoli R, Pessione E.: “The neuroendocrinological role of microbial glutamate and GABA signaling” Front. Syst. Microbiol. (2016) 7, 1934 DOI 10.3389/fmicb.2016.01934

Catucci G, Zgrablic I, Lanciani F, Valetti F, Minerdi D, Ballou DP, Gilardi G, Sadeghi SJ. Characterization of a new Baeyer-Villiger monooxygenase and conversion to a solely N-or S-oxidizing enzyme by a single R292 mutation. Biochim Biophys Acta. 2016 Sep;1864(9):1177-87. doi: 10.1016/j.bbapap.2016.06.010.

Castrignanò S, Valetti F, Gilardi G, Sadeghi SJ. Graphene oxide-mediated electrochemistry of glucose oxidase on glassy carbon electrodes. Biotechnol Appl Biochem. 2016 63, 157-62.

Heath GR, Li M, Polignano IL, Richens JL, Catucci G, O'Shea P, Sadeghi SJ, Gilardi G, Butt JN, Jeuken LJ. Layer-by-Layer Assembly of Supported Lipid Bilayer Poly-L-Lysine Multilayers. Biomacromolecules. (2016) 17, 324-35.

Morra S, Maurelli S, Chiesa M, Mulder DW, Ratzloff MW, Giamello E, King PW, Gilardi G, Valetti F. The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation. Biochim Biophys Acta. (2016) 1857, 98-106..

2015

Pessione A., Lo Bianco G., Mangiapane E., Cirrincione S., Pessione E.” Phenotypic characterization of potentially probiotic lactic acid bacteria isolated from olives: evaluation of short chain fatty acids and analysis of extracellular proteomes Food Res. Int. (2015) 67, 247-254.

Minerdi D, Zgrablic I, Castrignanò S, Catucci G, Medana C, Terlizzi ME, Gribaudo G, Gilardi G, Sadeghi SJ. Escherichia coli Overexpressing a Baeyer-Villiger Monooxygenase from Acinetobacter radioresistens Becomes Resistant to Imipenem. Antimicrob Agents Chemother. 2015 60, 64-74.

Di Nardo G, Dell'Angelo V, Catucci G, Sadeghi SJ, Gilardi G. Subtle structural changes in the Asp251Gly/Gln307His P450 BM3 mutant responsible for new activity toward diclofenac, tolbutamide and ibuprofen. Arch. Biochem. Biophys. 2015 pii: S0003-9861(15)30116-8.

Morra S, Cordara A, Gilardi G, Valetti F. Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase. Protein Sci. 2015 24, 2090-4.

Morra S, Valetti F, Sarasso V, Castrignanò S, Sadeghi SJ, Gilardi G. Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens. Bioelectrochemistry (2015) 106(Pt B): 258-62.

Noti O., Vaudano E., Pessione E., Garcia-Moruno E. Short-term response of different Saccharomyces cerevisiae strains to hyperosmotic stress caused by inoculation in grape must: RT-qPCR study and metabolite analysis. Food Microbiol. (2015), 52, 49-58.

Costantini A., Rantsiou K., Majumder A., Jacobsen S., Pessione E., Svensson B., Garcia-Moruno E., Cocolin L. Complementing DIGE proteomics and DNA subarray analyses to shed light on Oenococcus oeni adaptation to ethanol in wine-simulated conditions. J. Proteomics. (2015), 123, 114-127.

Porto B.A., Mangiapane E., Pessione A., Neves M.J., Pessione E., Martins F.S. Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis. J. Funct. Food. (2015), 17, 828-836.

Mangiapane E., Mazzoli R., Pessione A., Svensson B., Riedel K., Pessione E. (2015). Ten years of subproteome investigations in lactic acid bacteria: A key for food starter and probiotic typing. J. Proteomics (2015), doi:10.1016/j.jprot.2015.04.028.

Rua F, Sadeghi SJ, Castrignanò S, Valetti F, Gilardi G. Electrochemistry of Canis familiaris cytochrome P450 2D15 with gold nanoparticles: An alternative to animal testing in drug discovery. Bioelectrochemistry (2015) 105:110-116.

Castrignanò S, Valetti F, Gilardi G, Sadeghi SJ. Graphene oxide mediated electrochemistry of glucose oxidase on glassy carbon electrodes. Biotechnol. Appl. Biochem. (2015) doi: 10.1002/bab.1392.

Morra S, Mongili B, Maurelli S, Gilardi G, Valetti F. Isolation and characterisation of a new [FeFe]-hydrogenase from Clostridium perfringens. Biotechnol. Appl. Biochem. (2015) doi: 10.1002/bab.1382.

Castrignanò, S, Gilardi, G. and Sadeghi, S.J.  Human flavin-containing monooxygenase 3 on graphene oxide for drug metabolism screening Anal. Chem. (2015) 87, 2974-2980.

Di Nardo, G., Castrignanò, S., Sadeghi, S.J., Baravalle, R. and Gilardi, G. “Bioelectrochemistry as a tool for the study of aromatization of steroids by human aromatase”, Electrochem. Comm. (2015), 52: 25-28. DOI: 10.1016/j.elecom.2015.01.007.

Di Nardo G, Breitner M, Bandino A, Ghosh D, Jennings GK, Hackett JC, Gilardi G. Evidence for an elevated aspartate pKa in the active site of human aromatase.  J. Biol. Chem. (2015) 290, 1186-1196. DOI 10.1074/jbc.M114.595108.

Minerdi D, Sadeghi SJ, Di Nardo G, Rua F, Castrignanò S, Allegra P, Gilardi G. CYP116B5: a new class VII catalytically self-sufficient cytochrome P450 from Acinetobacter radioresistens that enables growth on alkanes.  Mol. Microbiol. (2014) doi: 10.1111/mmi.12883.

2014

Pessione A, Lo Bianco G, Mangiapane E, Cirrincione S, Pessione E. Characterization of potentially probiotic lactic acid bacteria isolated from olives: evaluation of short chain fatty acids production and analysis of the extracellular proteome. Food Res Int, 2014, doi:10.1016/j.foodres.2014.11.029.

Mazzoli R, Bosco F, Mizrahi I, Bayer EA, Pessione E. Towards lactic acid bacteria-based biorefineries. Biotech. Adv. (2014) 32: 1216-36.

Mangiapane E, Pessione A, Pessione E. Selenium and selenoproteins: an overview on different biological systems. Curr Protein Pept Sci. (2014) 15: 598-607.

Morra S, Arizzi, M, Allegra P, La Licata B, Sagnelli F, Zitella P, Gilardi G, Valetti F. Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant. Int. J. Hydrogen Energy (2014) 39, 9018-9027.

Selim S, Hagagy N, Aziz MA, El-Meleigy ES, Pessione E. Thermostable alkaline halophilic-protease production by Natronolimnobius innermongolicus WN18. Nat. Prod. Res. (2014) 28:1476-9

Castrignanò S, Ortolani A, Sadeghi SJ, Di Nardo G, Allegra P, and Gilardi G. Electrochemical detection of human cytochrome P450 2A6 inhibition: a step toward reducing dependence on smoking. Anal.Chem. (2014) 86, 2760-2766.

Mangiapane E, Lamberti C, Pessione A, Galano E, Amoresano A, Pessione E. Selenium effects on the metabolism of a Se-metabolizing Lactobacillus reuteri: analysis of envelope-enriched and extracellular proteomes. Mol. BioSyst. (2014) 10, 1272-1780.

Lamberti C, Genovese F, Coisson JD, Lo Bianco G, Cocolin LS, Pessione, E. Anti-S. aureus and anti-L. monocytogenes molecules produced by cheese-isolated lab. Czec.J.Food Sci. (2014) 32, 1, 54-60.

Riva Violetta M. Mazzoli R, Barello C, Fattori P, Giuffrida M,  Pessione E. Combining MS, PMF  and N-terminal amino acid sequencing for multiplexed characterization of a bacterial surfactant glycoprotein   RSC Adv. (2014) 4, 10918 – 10927.

Micalella C, Bruno, S, Mozzarelli A, Caglio R, Valetti F, Pessione E, Giunta C. Ormosil gels doped with engineered catechol 1,2 dioxygenases for chlorocatechols bioremediation. Biotechnol. Appl. Biochem. (2014) 61, 297-303.

Pessione A, Zapponi M, Mandili G, Fattori P, Mangiapane E, Mazzoli R, Pessione E. Enantioselective lactic acid production by an Enterococcus faecium strain showing potential in agro-industrial waste bioconversion: Physiological and proteomic studies. J Biotechnol. (2014) 173C:31-40.

2013

Mangiapane E, Lamberti C, Pessione A, Ceruti P, Novelli F, Galano E, Virkola R,  Korhonen TK, Pessione E. An integrated proteomic and physiological approach to understand the adhesion mechanism of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143 JiOMICS (2013) 3, 145-156.

Genovese F; Coïsson JD; Majumder A; Pessione A; Svensson B; Jacobsen S; Pessione E: An exoproteome approach to monitor safety of a cheese-isolated Lactococcus lactis Food Research International  (2013) 54,1072-1079.

Valetti F, Gilardi G. Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis. Biomolecules 2013, 3(4), 778-811.

Xia J, Giovannozzi AM, Sadeghi SJ, Gilardi G, Rossi AM. Laser-written nanoporous silicon diffraction gratings for biosensors. Appl Opt. (2013) 52: 8802-8808.

Di Nardo G, Breitner M, Sadeghi SJ, Castrignanò S, Di Venere A, Nicolai E, Mei G, Allegra P, Gilardi G. Dynamics and flexibility of human aromatase probed by FTIR and time resolved fluorescence spectroscopy. Plos One (2013) 8(12):e82118..

Lo J, Di Nardo G, Griswold J, Egbuta C, Jiang W, Gilardi G, Ghosh D. Structural basis for the functional roles of critical residues in human cytochrome p450 aromatase. Biochemistry (2013) 52, 5821-5829.

Catucci G, Occhipinti A, Maffei M, Gilardi G, Sadeghi SJ. Effect of human flavin-containing monooxygenase 3 polymorphism on the metabolism of aurora kinase inhibitors. Int. J. Mol. Sci. (2013) 14, ,2707-16.

Costantini A, Pietroniro R, Doria F, Pessione E, Garcia-Moruno E. Putrescine production from different amino acid precursors by lactic acid bacteria from wine and cider.  Int. J. Food Microbiol. (2013) 165, 11-17.

Galano E, Mangiapane E, Bianga J, Palmese A, Pessione E, Szpunar J, Lobinski R, Amoresano A. Privileged incorporation of selenium as selenocysteine in Lactobacillus reuteri proteins demonstrated by selenium-specific imaging and proteomics.  Mol. Cell Proteomics. (2013) 12, 2196-2104.

Tsotsou GE, Di Nardo G, Sadeghi SJ, Fruttero R, Lazzarato L, Bertinaria M, Gilardi G. A rapid screening for cytochrome P450 catalysis on new chemical entities: cytochrome P450 BM3 and 1,2,5-oxadiazole derivatives. J. Biomol. Screen. (2013) 18, 211-8.

Di Nardo, G. and Gilardi, G. “Human aromatase: perspectives in biochemistry and biotechnology”, Biotechnology and Applied Biochemistry (2013), 60(1), 92-101.

Sadeghi, S.J. and Gilardi, G. “Chimeric P450 Enzymes: activity of artificial redox fusions driven by different reductases for biotechnological applications”, Biotechnology and Applied Biochemistry (2013) 60, 102-110.

Sideri, A., Goyal, A., Di Nardo, G., Tsotsou, G.E. and Gilardi, G. "Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution", Journal of Inorganic Biochemistry, (2013) 120, 1-7.

Caglio, R., Pessione, E., Valetti, F., Giunta, C., Ghibaudi, E. An EPR and thermostability investigation of wt and  

mutant forms of catechol 1,2 dioxygenase from Acinetobacter radioresistens S13.  Biometals (2013) 26(1):75-84.

2012

Di Nardo G, Gilardi G. Optimization of the Bacterial Cytochrome P450 BM3 System for the Production of Human Drug Metabolites.  Int. J. Mol. Sci. (2012) 13(12), 15901-15924.

Rua F, Di Nardo G, Sadeghi SJ, Gilardi G Towards reduction in animal sacrifice for drugs:Molecular modelling of Macaca fascicularis P450 2C20 for virtual screening of Homo sapiens P450 2C8 substrates  Biotechnol. Appl. Biochem. (2012) 59(6), 479-489.

Morra S, Giraudo A, Di Nardo G, King PW, Gilardi G, Valetti F. (2012) Site Saturation Mutagenesis Demonstrates

a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase. PLoS ONE (2012)

7(10): e48400.

Castrignanò S, Sadeghi SJ, Gilardi G. Entrapment of human flavin-containing monooxygenase 3 in the presence of gold nanoparticles: TEM, FTIR and electrocatalysis. Biochim Biophys Acta (2012) 1820(12):2072-8. .

Minerdi D, Zgrablic I, Sadeghi SJ, Gilardi G. Identification of a novel Baeyer-Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA. Microb Biotechnol., (2012), 5, 700-716.

Rua F, Sadeghi SJ, Castrignanò S, Di Nardo G, Gilardi G. Engineering Macaca fascicularis cytochrome P450 2C20 to reduce animal testing for new drugs. J. Inorg. Biochem., 2012, doi.org/10.1016/j.jinorgbio.2012.05.017

Sadeghi SJ, Ferrero S, Di Nardo G, Gilardi G. Drug-drug interactions and cooperative effects detected in electrochemically driven human cytochrome P450 3A4. Bioelectrochemistry, 2012, 86, 87-91.

Pessione E. Lactic acid bacteria contribution to gut microbiota complexity: lights and shadows. Front. Cell Infect. Microbiol. (2012), 2, 86.

Pessione A, Lamberti C, Cocolin L, Campolongo S, Grunau A, Giubergia S, Eberl L, Riedel K, Pessione E. Different protein expression profiles in cheese and clinical isolates of Enterococcus faecalis revealed by proteomic analysis. Proteomics, (2012), 12, 431-47.

Tsotsou, G.E., Sideri, A., Goyal, A., Di Nardo, G. and Gilardi G. Identification of mutant Asp251Gly Gln307His

of cytochrome P450 BM3 for the generation of metabolites of diclofenac, ibuprofen and tolbutamide. Chemistry, (2012), 18, 3582-8.

Ferrero, V.E., Di Nardo, G., Catucci, G., Sadeghi, S.J., Gilardi, G. Fluorescencedetection of ligand binding to labeled cytochrome P450 BM3. Dalton Trans. (2012), 41, 2018-2025.

Catucci, G., Gilardi, G., Jeuken, L., Sadeghi, S.J. In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3. Biochem. Pharmacol. (2012), 83, 551-558.

Valetti, F., Fantuzzi, A., Sadeghi, S.J., Gilardi, G. Iron-based redox centres of reductase and oxygenase components of phenol hydroxylase from A. radioresistens: a redox chain working at highly positive redox potentials. Metallomics. (2012), 4, 72-77.

Mazzoli, R., Lamberti, C., Pessione, E. Engineering new metabolic capabilities in bacteria: lessons from recombinant cellulolytic strategies. Trends Biotechnol. (2012), 30, 111-9.

2011

Morra, S., Valetti, F., Sadeghi, S.J., King, P.W., Meyer, T. and Gilardi, G. “Direct electrochemistry of an [FeFe]-hydrogenase on TiO2 electrode”, Chem Comm. (2011), 47, 10566-10568.

Giovannozzi, A.M., Ferrero, V.E.V., Pennecchi, F., Sadeghi, S.J., Gilardi, G. and  Rossi, A.M. “P450-based porous silicon biosensor for arachidonic acid detection”. Biosensors and Bioelectronics (2011), 28, 320-325

Maurelli, S., Chiesa, M., Giamello, E., Di Nardo, G., Ferrero, V.E., Gilardi, G. van Doorslaer, S. “Direct Spectroscopic Evidence for Binding of Anastrozole to the Iron Heme of Human Aromatase. Peering into the Mechanism of Aromatase Inhibition”. Chem Comm. (2011), 47, 10737–10739

Mazzoli, R., Fattori, P., Lamberti, C., Giuffrida, M.G., Zapponi, M., Giunta, C., and Pessione E. "High isoelectric point sub-proteome analysis of Acinetobacter radioresistens S13 reveals envelope stress responses induced by aromatic compounds" Mol. Biosyst. (2011), 7, 598-607.

Lamberti, C., Purrotti, M., Mazzoli, R., Fattori, P., Barello, C., Coïsson, J.D., Giunta, C., and Pessione, E. "ADI pathway and histidine decarboxylation are reciprocally regulated in Lactobacillus hilgardii ISE 5211: proteomic evidence" Amino Acids (2011), 41, 517-27.

Lamberti, C., Mangiapane, E., Pessione, A., Mazzoli, R., Giunta C., and Pessione E. "Proteomic characterization of a selenium-metabolizing probiotic Lactobacillus reuteri Lb2 BM for nutraceutical applications", Proteomics (2011) 2011, 11, 2212-21.

Micalella, C., Martignon, S., Bruno, S., Pioselli, B., Caglio, R., Valetti, F., Pessione, E., Giunta, C., and Rizzi, M. "X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: A multidisciplinary characterization of catechol 1,2 dioxygenase". Biochim. Biophys. Acta. (2010) 1814(6):817-23

Fantuzzi, A., Mak L.H., Capria E., Dodhia, V.R., Panicco, P., Collins. S. and Gilardi, G. “A new standardised electrochemical array for drug metabolic profiling with human cytochromes P450". Anal. Chem., (2011), 83, 3831-9.

Panicco, P., Dodhia, V.R., Fantuzzi, A. and Gilardi, G. “"First enzyme-based amperometric platform to determine the polymorphic response in drug metabolism by cytochromes P450". (2011), Anal. Chem., 83, 2179-2186.

Sadeghi, S.J., Fantuzzi, A. and Gilardi, G.  “Breakthrough in P450 bioelectrochemistry and future perspectives”, (2011), BBA - Proteins and Proteomics, 1814, 237-248.

Degregorio, D., Sadeghi, S.J., Di Nardo, G., Gilardi, G. and Solinas, S.P. “Understanding uncoupling in the multi-redox centre P450 3A4-BMR model system” (2011), J Biol Inorg Chem, 16, 109-116.

2010

Fantuzzi A., Capria E., Mak L.H., Dodhia V.R., Sadeghi S.J., Collins S., Somers G., Huq E., Gilardi G. “An electrochemical microfluidic platform for human P450 drug metabolism profiling” (2010) Anal. Chem., 82, 10222-10227.

Castrignanò, S., Sadeghi, S.J. and Gilardi, G. “Electro-catalysis by immobilised human flavin-containing monooxygenase isoform 3 (hFMO3), Anal Bioanal Chem, (2010), 398 (3), 1403-1409.

Mak, L.H., Sadeghi, S.J., Fantuzzi, A. and Gilardi, G.  “Control of Human Cytochrome P450 2E1 Electrocatalytic Response as a Result of Unique Orientation on Gold Electrodes”, Anal.Chem. (2010), 82 (12), 5357-5362.   

Sadeghi SJ, Meirinhos R, Catucci G, Dodhia VR, Di Nardo G and Gilardi G. “Direct electrochemistry of drug metabolizing human flavin-containing monooxygenase: electrochemical turnover of benzydamine and tamoxifen”, J Am Chem Soc. (2010), 132(2), 458-9.

Mazzoli, R., Pessione, E., Dufour, M., Laroute, V., Giuffrida, M.G., Giunta, C., Cocaign-Bousquet, M., and Loubière, P. "Glutamate-induced metabolic changes in Lactococcus lactis NCDO 2118 during GABA production: combined transcriptomic and proteomic analysis" Amino Acids (2010), 39 (3), 727-737.

Pessione, A., Lamberti, C., and Pessione, E. "Proteomics as a tool for studying energy metabolism in lactic acid bacteria" Mol. Biosyst. (2010), 6 (8),1419-1430.

2009

Di Nardo, G., Roggero, C., Campolongo, S., Valetti, F., Trotta, F. and Gilardi, G. “Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges”, Dalton Transactions, (2009), 33, 6507 - 6512

Fantuzzi, A., Meharenna, Y.T., Briscoe, P.B., Guelesquin, F., Sadeghi, S.J. and GilardI “Characterisation of the electron transfer and complex formation between Flavodoxin from D. vulgaris and the heam domain of Cytochrome P450 BM3 from B. megaterium”, BBA – Bioenergetics (2009), 1787 (4), 234-241

Di Nardo, G., Di Venere, A., Mei, G., Sadeghi, S.J., Wilson, J.R., and Gilardi, G. “Engineering heme binding sites in monomeric rop”, J Biol Inorg Chem (2009), 14 (4), 497-505

Fantuzzi, A., Artali, R., Bombieri. G., Marchini, M., Meneghetti, F., Gilardi, G., Sadeghi, S. J., Cavazzini, D., G.L. Rossi, “Redox properties and crystal structures of a D. vulgaris flavodoxin mutant in the monomeric and homodimeric forms” BBA - Proteins and Proteomics (2009) 1794 (3), 496-505

Zava, S., Barello, C., Pessione, A., Garoffo, L.P., Fattori, P., Montorfano, G., Conti, A., Giunta, C., Pessione, E., Berra, B., and Giuffrida, M.G. "Mare's colostrum globules stimulate fibroblast growth in vitro: a biochemical study" J.Med.Food. (2009), 12 (4), 836-845

Pessione, E., Pessione, A., Lamberti, C., Coïsson, D.J., Riedel, K., Mazzoli, R., Bonetta, S., Eberl, L., and Giunta, C. "First evidence of a membrane-bound, tyramine and beta-phenylethylamine producing, tyrosine decarboxylase in Enterococcus faecalis: a two-dimensional electrophoresis proteomic study" Proteomics (2009), 9 (10), 2695-2710

Caglio, R., Valetti, F., Caposio, P., Gribaudo, G., Pessione, E., and Giunta, C. "Fine-tuning of catalytic properties of catechol 1,2-dioxygenase by active site tailoring" Chembiochem. (2009), 10 (6),1015-1024

Mazzoli, R., Lamberti, C., Coisson, J.D., Purrotti, M., Arlorio, M., Giuffrida, M.G., Giunta, C., and Pessione, E. "Influence of ethanol, malate and arginine on histamine production of Lactobacillus hilgardii isolated from an Italian red wine", Amino Acids (2009), 36 (1), 81-89

Antoniotti, S., Fattori, P., Tomatis, C., Pessione, E., and Munaron, L. "Arachidonic acid and calcium signals in human breast tumor-derived endothelial cells: a proteomic study" J. Recept. Signal. Transduct. Res. (2009), 29 (5), 257-265